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fitc streptavidin  (Thermo Fisher)


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    Structured Review

    Thermo Fisher fitc streptavidin
    Fitc Streptavidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/streptavidin+fitc/Streptavidin/pmc12955099-73-52-55
    Average 99 stars, based on 1 article reviews
    fitc streptavidin - by Bioz Stars, 2026-09
    99/100 stars

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    Incubation:

    Article Title: The TDG protein environment connects active DNA demethylation with chromatin and RNA biology.
    Article Snippet: .. Subsequently, cells were blocked with 3% BSA in PBS-T for 1 h and incubated 337 with antibodies against Nucleolin (ab134164, 1:500) or HA (Cell Signaling C29F4, 1:800) at 338 RT for 1 h, followed by incubation with Streptavidin-FITC (ThermoFischer SA1001, 1:1’000) 339 and secondary antibody (Alexa-647 or Alexa-596, Invitrogen 1:500) at RT in the dark for 1 h. 340 After three washing steps with PBS-T, cells were incubated with DAPI diluted in PBS (200 341 ng/mL), washed with PBS and water, and mounted on slides with VectaShield (Vector 342 Laboratories). .. For Proximity ligation assay: staining was performed as recommended by the 343 manufacturer (Duolink®, Sigma-Aldrich).

    Article Title: The TDG protein environment connects active DNA demethylation with chromatin and RNA biology
    Article Snippet: .. Subsequently, cells were blocked with 3% BSA in PBS-T for 1 h and incubated with antibodies against Nucleolin (ab134164, 1:500) or HA (Cell Signaling C29F4, 1:800) at RT for 1 h, followed by incubation with Streptavidin-FITC (ThermoFischer SA1001, 1:1′000) and secondary antibody (Alexa-647 or Alexa-596, Invitrogen 1:500) at RT in the dark for 1 h. After three washing steps with PBS-T, cells were incubated with DAPI diluted in PBS (200 ng/mL), washed with PBS and water, and mounted on slides with VectaShield (Vector Laboratories). .. For Proximity ligation assay: staining was performed as recommended by the manufacturer (Duolink®, Sigma-Aldrich).

    Staining:

    Article Title: Tumor Cell Clustering Enhances Metastatic Competence by Regulating the H3K36 Histone Demethylase KDM2A
    Article Snippet: All samples were mounted in Prolong Gold Antifade Reagent with DAPI (Invitrogen cat. P36935). .. For junctional staining, cells were labeled for 30 minutes at 37°C with 0.8mM NHS-Biotin (Thermo Scientific cat. A39256) in PBS prior to collection and incubating with 1:200 Streptavidin-FITC (Invitrogen SA1000) for 2-3h at room temperature. .. Clusters were imaged on a Keyence microscope using phase contrast microscopy and fluorescent detection where possible.

    Labeling:

    Article Title: Tumor Cell Clustering Enhances Metastatic Competence by Regulating the H3K36 Histone Demethylase KDM2A
    Article Snippet: All samples were mounted in Prolong Gold Antifade Reagent with DAPI (Invitrogen cat. P36935). .. For junctional staining, cells were labeled for 30 minutes at 37°C with 0.8mM NHS-Biotin (Thermo Scientific cat. A39256) in PBS prior to collection and incubating with 1:200 Streptavidin-FITC (Invitrogen SA1000) for 2-3h at room temperature. .. Clusters were imaged on a Keyence microscope using phase contrast microscopy and fluorescent detection where possible.



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